Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death

Garnacho-Montero, José and Palacios-García, Inmaculada and Díaz-Martín, Ana and Gutiérrez-Pizarraya, Antonio and López-Sánchez, José M. and Gómez, Elísabet Alcocer and Cordero, Mario D. UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es (2020) Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. Shock, 54 (3). pp. 294-300. ISSN 1073-2322

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Abstract

Introduction: Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat–containing receptor, pyrin domain–containing-3 (NLRP3), and serum interleukin-1β (IL-1 β) in critically ill patients, their changes over the first week and their prognostic value in septic patients. Methods: Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1β as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors). Results: Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 β and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died. Conclusions: NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive.

Item Type:	Article
Commentary on:	Eprints 0 not found.
Uncontrolled Keywords:	Caspase-1; Caspase-3; inflammation ; Mortality; NLRP3 inflammasome NLRP3 inflammasome; Sepsis.
Subjects:	Subjects > Biomedicine
Divisions:	Europe University of Atlantic > Research > Scientific Production
SWORD Depositor:	Users 0 not found.
Depositing User:	Sr Bibliotecario
Date Deposited:	20 May 2021 11:03
Last Modified:	03 Jul 2023 10:35
URI:	http://repositorio.funiber.org/id/eprint/119

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• Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. (deposited 20 May 2021 11:03) [Currently Displayed]

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• Garnacho-Montero, José and Palacios-García, Inmaculada and Díaz-Martín, Ana and Gutiérrez-Pizarraya, Antonio and López-Sánchez, José M. and Gómez, Elísabet Alcocer and Cordero, Mario D. Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. (deposited 20 May 2021 11:03) [Currently Displayed]

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