Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation

Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Gasparrini, Massimiliano and Afrin, Sadia and Mazzoni, Luca and Cordero, Mario and Mezzetti, Bruno and Quiles, José L. and Battino, Maurizio tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. Nutrients, 9 (6). p. 621. ISSN 2072-6643

Preview

Text nutrients-09-00621-v2.pdf - Published Version Available under License Creative Commons Attribution. Download (4MB) | Preview

Abstract

Regulation of lipid metabolism is essential for treatment and prevention of several chronic diseases such as obesity, diabetes, and cardiovascular diseases, which are responsible for most deaths worldwide. It has been demonstrated that the AMP-activated protein kinase (AMPK) has a direct impact on lipid metabolism by modulating several downstream-signaling components. The main objective of the present work was to evaluate the in vitro effect of a methanolic strawberry extract on AMPK and its possible repercussion on lipid metabolism in human hepatocellular carcinoma cells (HepG2). For such purpose, the lipid profile and the expression of proteins metabolically related to AMPK were determined on cells lysates. The results demonstrated that strawberry methanolic extract decreased total cholesterol, low-density lipoprotein (LDL)-cholesterol, and triglycerides levels (up to 0.50-, 0.30-, and 0.40-fold, respectively) while it stimulated the p-AMPK/AMPK expression (up to 3.06-fold), compared to the control. AMPK stimulation led to the phosphorylation and consequent inactivation of acetyl coenzyme A carboxylase (ACC) and inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the major regulators of fatty acids and cholesterol synthesis, respectively. Strawberry treatment also entailed a 4.34-, 2.37-, and 2.47-fold overexpression of LDL receptor, sirtuin 1 (Sirt1), and the peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α), respectively, compared to control. The observed results were counteracted by treatment with compound C, an AMPK pharmacological inhibitor, confirming that multiple effects of strawberries on lipid metabolism are mediated by the activation of this protein.

Item Type:	Article
Commentary on:	Eprints 0 not found.
Uncontrolled Keywords:	Strawberry; Cholesterol synthesis; Fatty acids synthesis; Hypolipidemic agent.
Subjects:	Subjects > Biomedicine
Divisions:	Europe University of Atlantic > Research > Scientific Production Ibero-american International University > Research > Scientific Production
SWORD Depositor:	Users 0 not found.
Depositing User:	Sr Bibliotecario
Date Deposited:	20 May 2021 10:57
Last Modified:	08 Jul 2021 22:49
URI:	http://repositorio.funiber.org/id/eprint/135

Available Versions of this Item

• Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. (deposited 20 May 2021 10:57) [Currently Displayed]

Commentary/Response Threads

• Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Gasparrini, Massimiliano and Afrin, Sadia and Mazzoni, Luca and Cordero, Mario and Mezzetti, Bruno and Quiles, José L. and Battino, Maurizio Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. (deposited 20 May 2021 10:57) [Currently Displayed]

Actions (login required)

View Item