Elexpuru Zabaleta, Maria and Lazzarini, Raffaella and Tartaglione, Maria Fiorella and Piva, Francesco and Ciarapica, Veronica and Marinelli Busilacchi, Elena and Poloni, Antonella and Valentino, Matteo and Santarelli, Lory and Bracci, Massimo maria.elexpuru@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2023) A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure. Molecular Biology Reports. ISSN 0301-4851
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Abstract
Background The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. Methods and results The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time.
| Item Type: | Article |
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| Uncontrolled Keywords: | Extremely low frequency magnetic feld; Breast cancer; Cell viability; MDA-MB-231; MCF-7; MCF-10A |
| Subjects: | Subjects > Biomedicine |
| Divisions: | Europe University of Atlantic > Research > Scientific Production |
| Depositing User: | Sr Bibliotecario |
| Date Deposited: | 21 Nov 2022 09:29 |
| Last Modified: | 10 Jul 2023 11:11 |
| URI: | http://repositorio.funiber.org/id/eprint/4691 |
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