MacDonald, Lewis and Keenan, Sean and Di Lorenzo, Flaviana and Adade, Nana E. and Kenna, Dervla T. D. and Millar, Beverley C. and Moore, John E. and Ramos Vivas, Jose and Molinaro, Antonio and Valvano, Miguel A. and Avci, Fikri Y. UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.ramos@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Polymyxin Resistance and Heteroresistance Are Common in Clinical Isolates of Achromobacter Species and Correlate with Modifications of the Lipid A Moiety of Lipopolysaccharide. Microbiology Spectrum. ISSN 2165-0497
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Abstract
The Achromobacter genus includes opportunistic pathogens that can cause chronic infections in immunocompromised patients, especially in people with cystic fibrosis (CF). Treatment of Achromobacter infections is complicated by antimicrobial resistance. In this study, a collection of Achromobacter clinical isolates, from CF and non-CF sources, was investigated for polymyxin B (PmB) resistance. Additionally, the effect of PmB challenge in a subset of isolates was examined and the presence of PmB-resistant subpopulations within the isolates was described. Further, chemical and mass spectrometry analyses of the lipid A of Achromobacter clinical isolates enabled the determination of the most common structures and showed that PmB challenge was associated with lipid A modifications that included the addition of glucosamine and palmitoylation and the concomitant loss of the free phosphate at the C-1 position. This study demonstrates that lipid A modifications associated with PmB resistance are prevalent in Achromobacter and that subresistant populations displaying the addition of positively charged residues and additional acyl chains to lipid A can be selected for and isolated from PmB-sensitive Achromobacter clinical isolates.
| Item Type: | Article |
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| Uncontrolled Keywords: | Achromobacter, antibiotic resistance, lipid A, opportunistic infections, polymyxins |
| Subjects: | Subjects > Biomedicine |
| Divisions: | Europe University of Atlantic > Research > Scientific Production |
| Depositing User: | Sr Bibliotecario |
| Date Deposited: | 19 Dec 2022 09:18 |
| Last Modified: | 17 Jul 2023 07:31 |
| URI: | http://repositorio.funiber.org/id/eprint/5100 |
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