Giampieri, Francesca and Alvarez-Suarez, José and Mazzoni, Luca and Forbes-Hernandez, Tamara Y. and Gasparrini, Massimiliano and Gonzàlez-Paramàs, Ana and Santos-Buelga, Celestino and Quiles, José and Bompadre, Stefano and Mezzetti, Bruno and Battino, Maurizio UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2014) Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality. Molecules, 19 (6). pp. 7798-7816. ISSN 1420-3049
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Abstract
Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | strawberry; anthocyanin; ROS; lipid peroxidation; DNA damage; fibroblasts |
| Subjects: | Subjects > Nutrition |
| Divisions: | Europe University of Atlantic > Research > Scientific Production Ibero-american International University > Research > Scientific Production |
| Depositing User: | Sr Bibliotecario |
| Date Deposited: | 13 Apr 2022 08:07 |
| Last Modified: | 13 Apr 2022 08:07 |
| URI: | http://repositorio.funiber.org/id/eprint/612 |
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