Greco, Stefania and Pellegrino, Pamela and Giampieri, Francesca and Capocasa, Franco and Delli Carpini, Giovanni and Battino, Maurizio and Mezzetti, Bruno and Giannubilo, Stefano Raffaele and Ciavattini, Andrea and Ciarmela, Pasquapina UNSPECIFIED, UNSPECIFIED, francesca.giampieri@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2023) The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells. Nutrients, 15 (11). p. 2557. ISSN 2072-6643
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Abstract
Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 µg/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson’s trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | leiomyosarcoma; phytochemicals; strawberry; 3D culture |
| Subjects: | Subjects > Nutrition |
| Divisions: | Europe University of Atlantic > Research > Scientific Production |
| Depositing User: | Sr Bibliotecario |
| Date Deposited: | 31 May 2023 10:37 |
| Last Modified: | 21 Oct 2024 12:09 |
| URI: | http://repositorio.funiber.org/id/eprint/7353 |
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