Giampieri, Francesca and Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Mazzoni, Luca and Capocasa, Franco and Sabbadini, Silvia and Alvarez-Suarez, José M. and Afrin, Sadia and Rosati, Carlo and Pandolfini, Tiziana and Molesini, Barbara and Sánchez-Sevilla, José Federico and Amaya, Iraida and Mezzetti, Bruno and Battino, Maurizio UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2018) Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. Journal of Agricultural and Food Chemistry, 66 (3). pp. 581-592. ISSN 0021-8561
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Abstract
Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.
| Item Type: | Article |
|---|---|
| Commentary on: | Eprints 0 not found. |
| Uncontrolled Keywords: | Anthocyanidin synthase; Anticancer effects; Apoptosis; Flavonoids; RNA-seq. |
| Subjects: | Subjects > Nutrition |
| Divisions: | Europe University of Atlantic > Research > Scientific Production |
| SWORD Depositor: | Users 0 not found. |
| Depositing User: | Sr Bibliotecario |
| Date Deposited: | 20 May 2021 10:38 |
| Last Modified: | 02 Mar 2022 15:13 |
| URI: | http://repositorio.funiber.org/id/eprint/87 |
Available Versions of this Item
- Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. (deposited 20 May 2021 10:38) [Currently Displayed]
Commentary/Response Threads
- Giampieri, Francesca and Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Mazzoni, Luca and Capocasa, Franco and Sabbadini, Silvia and Alvarez-Suarez, José M. and Afrin, Sadia and Rosati, Carlo and Pandolfini, Tiziana and Molesini, Barbara and Sánchez-Sevilla, José Federico and Amaya, Iraida and Mezzetti, Bruno and Battino, Maurizio Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. (deposited 20 May 2021 10:38) [Currently Displayed]
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